| 1. Soluble recombinant protein with GST tag for the separation and purification, dissolved in high concentrations of denaturant inclusion body proteins can not be purified using this medium; specificity, the GST fusion protein with high affinity from bacterial brokenliquid step affinity purification can be obtained in a purity of more than 95% of the target protein; 3 on the medium-bound GST fusion protein, the solution of high concentrations of reduced glutathione (GSH) (33 mM) competition sexualelution. Different GST fusion protein is no need to fumble best of GSH elution concentration, make medium use more simple; 4. Renewable, and repeated use. |
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