| 1. Compared with the tetravalent metal ion chelating agent is nitrilotriacetic acid made ??of Ni-NTA-agarose, Ni-IDA-agarose with the target protein the ability to bind stronger, larger capacity of the column; 2.(8M urea, 6M guanidine hydrochloride) in the performance and stability, and can be directly used for the separation and purification of the inclusion body protein; 3 support denatured inclusion body protein renatured, played separation, renaturation is carried out simultaneously the effects of the high concentrations of denaturing agents; 3 to 5 times a matrix (4) can be repeated use and regeneration; with Ni2 + highly cross-linked 6% agarose; the Average Particle Size:90um; pH Stability:3 ~ 12. |
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