Order NO.: BSP079-4 | 品牌 : BBI |
英文名 | Ni-NTA 5 ml(Pre-Packed Gravity Column) | |
别名 | C600793-0010 | |
储存 | 2~8°C | |
相关类别 | 蛋白纯化与层析 |
产品编号 | 新编号 | 包装规格 | 库存 | 目录价(元) | 网购价(元) | 订购数量 |
BSP079-3 | C600793-0005 | 5 EA | 有 | 2300.0 | 1840.0 | |
BSP079-4 | C600793-0010 | 10 EA | 有 | 4500.0 | 3600.0 | |
大包装请询 |
Features |
1. And trivalent metal ion chelating agent is iminodiacetic acid made ??of Ni-IDA-agarose compared to non-specific adsorption of the Ni-NTA-agarose was significantly reduced, allows the user to obtain a higher purity of the targetprotein 2. support denatured inclusion body protein renatured, played separation, synchronized with the effect of refolding 3 can withstand a higher concentration of the solution a reducing reagent (β-mercaptoethanol to 20 mM highest available). 4 agarose matrix remain stable in the range of pH 3 to 12 and can withstand repeated reign cleaning (CIP). 5 purification process minimal Ni2 + leakage 6 can be repeated use and regeneration of 5 to 10 times. |
General Description |
Matrix: with Ni2 + highly cross-linked 6% agarose; the Average Particle Size: 90 um; Dynamic Binding Capacity: Break THROUGH Capacity AT 10%, to 150 cm / h, about 40 mg histidine-tagged protein / mlFiller;metal Ion load: about 15 μmol、70% Acetic acid30 min, 30% 2-异丙醇1 h, 2% SDS; pH Stability: 3~12. |
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