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Order NO.: BSP079-4 品牌 : BBI
Ni-NTA 5 ml(预装重力柱)
英文名 Ni-NTA 5 ml(Pre-Packed Gravity Column)
别名 C600793-0010
储存 2~8°C
相关类别 蛋白纯化与层析
产品编号 新编号 包装规格 库存 目录价(元) 网购价(元) 订购数量
BSP079-3 C600793-0005 5 EA 2300.0 1840.0
BSP079-4 C600793-0010 10 EA 4500.0 3600.0
      大包装请询
Features
1. And trivalent metal ion chelating agent is iminodiacetic acid made ??of Ni-IDA-agarose compared to non-specific adsorption of the Ni-NTA-agarose was significantly reduced, allows the user to obtain a higher purity of the targetprotein 2. support denatured inclusion body protein renatured, played separation, synchronized with the effect of refolding 3 can withstand a higher concentration of the solution a reducing reagent (β-mercaptoethanol to 20 mM highest available). 4 agarose matrix remain stable in the range of pH 3 to 12 and can withstand repeated reign cleaning (CIP). 5 purification process minimal Ni2 + leakage 6 can be repeated use and regeneration of 5 to 10 times.
General Description
Matrix: with Ni2 + highly cross-linked 6% agarose; the Average Particle Size: 90 um; Dynamic Binding Capacity: Break THROUGH Capacity AT 10%, to 150 cm / h, about 40 mg histidine-tagged protein / mlFiller;metal Ion load: about 15 μmol、70% Acetic acid30 min, 30% 2-异丙醇1 h, 2% SDS; pH Stability: 3~12.
Properties

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