Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of degrading the proteins. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. This series of protease inhibitor cocktail contain a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases etc., which have been optimized and tested for bacterial, fungal /yeast, plant and mammalian cell protein extraction. |