| The HOOK? HRP PLUS labeling kit is a high efficiency enzyme labeling kit for tagging proteins with horseradish peroxidase enzyme. This kit has an activated HRP that couples with high efficiency (>90%) to the numerous amine groups of proteins and is superior to the commonly used glutaraldehyde coupling chemistry. This kit uses HOOK? HRP PLUS, which is HRP that has been activated by the addition of reactive aldehydes. The aldehyde groups react spontaneously and at high efficiency with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by the supplied reduction agent. Following quenching of the reaction the protein is linked to the horseradish peroxidase enzyme by stable amine linkage. The labeled protein, or antibody, can now be used for immunoblotting, ELISA and histochemical techniques. This kit is supplied with JAW? Dry Buffer packs for convenient PBS and Carbonate/ Bicarbonate buffer preparation.Features:Activity is 120~200 units/mg; Reacts with primary amines to form covalent amine bonds; Stable for >12 months at -20°C; High consistency. |
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