Order NO.: PB0452-1ml | 品牌 : BBI |
英文名 | Proteinase K (Recombinant), 10 mg/ml, ~600 U/ml | |
别名 | B600452-0001 | |
储存 | -20°C | |
相关类别 | DNA修饰酶 |
产品编号 | 新编号 | 包装规格 | 库存 | 目录价(元) | 网购价(元) | 订购数量 |
PB0452-1ml | B600452-0001 | 1 ml | 有 | 210.0 | 168.0 | |
大包装请询 |
Unit definition |
One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine in 1 min at 37°C using denatured hemoglobin as substrate. |
Applications |
Isolation of genomic DNA from mouse tail Isolation of genomic DNA from cultured cells Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines Determination of enzyme localization Improving cloning efficiency of PCR products |
Features |
Ready-to-use solution Active in a wide range of reaction conditions |
General Description |
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease ). The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide. |
Properties |
Quality control | The absence of endo-, exodeoxyribonucleases, and ribonucleases confirmed by appropriate quality tests. |
Source | Pichia pastoris cells with a cloned gene encoding Tritirachium album endolytic protease (Proteinase K). |