Buffer RLT should be stored at 2~8°C and the others are stored at room temperature. Proteinase K solution remain stable at room temperature for 6 months, store at 2-8°C for long term.
Purified RNA is eluted in RNase-free water has OD260/OD280 ratios of 1. 9-2. 1 (measured in 10 mM Tris-Cl, pH 7. 5) and is ideal for use in most downstream applications including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.
Features
1. Fast. Using a rapid spin column format, the whole procedure takes less than 20 minutes. 2. High Quality of RNA. Purified RNA has an OD260/OD280 ratio of 1.9-2.0. 3. Versatile. Suitable for purification of total RNA from various samples such as bacteria, fungi, animals and some of plants. 4. Economic.
General Description
The EZ-10 Total RNA Mini-Preps Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format. Biological samples are first lysed and homogenized in denaturing guanidine-containing buffer (Buffer RLT), which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact RNA. RNA in the whole homogenate is selectively absorbed on EZ-10 Spin Column, all protein and other components are removed in the flow-through. Remaining contaminants and salts are efficiently washed away.
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