BEP0117,T7 RNA Polymerase

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Order NO.: BEP0117

品牌 : BBI

T7 RNA Polymerase

Unit definition

One unit is the amount of enzyme required to incorporate 1 nmol NTP into an acid - insoluble material in 1 hour at 37°C.

Properties

Quality Control	Purified free of contaminating endonucleases and exonucleases.
Storage conditions	50 mM Tris - HCl (pH 7.5); 100 mM NaCl; 20 mM 2-mercaptoethanol; 1 mM EDTA; 0.1% Triton X-100; 50% glycerol. Store at -20°C.
Optimal temperature	37°C
Optimal buffer	SE-buffer RNA polymerase T7, (50 mM Tris-HCl (pH 7.5 at 25°C); 6 mM MgCl2; 10 mM Dithiothreitol; 2 mM spermidine.)
Source	Isolated from E.coli strain that carries the cloned T7 gene I