| Campatible Ends | Asp718I , KpnI^ |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Blocked by overlapping Dcm methylation(CmCWGG):GGTACCWGG. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. |
| Ligations | After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Optimal temperature | 37°C |
| Optimal buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Assayed on | Lambda DNA(dcm-) |
| Source | Acinetobacter calcoaceticus 65 |
| Recognition sites | G↑GTACC |
021-37772169 800-820-1016 400-821-0268 
021-37772170 
sales@sangon.com