| Campatible Ends | BsrBI, MbiI |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Methylation sensitivity not tested |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. |
| Ligations | After 5-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 50% can be recut. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Optimal temperature | 37°C |
| Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Assayed on | Lambda DNA |
| Source | Acinetobacter calcoaceticus BS |
| Recognition sites | GAG↑CGG |
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