| Campatible Ends | Psp1406I |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Blocked by CG methylation |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. |
| Ligations | After 2-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 0.05% Triton X-100; 50% glycerol; Store at -20°C. |
| Optimal temperature | 37°C |
| Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA |
| Assayed on | Lambda DNA |
| Source | Acinetobacter calcoaceticus |
| Recognition sites | AA↑CGTT |
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