| Campatible Ends | ApoI, XapI |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Methlation effect | Methylation sensitivity not tested |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 50 u.a. of enzyme for 16 hours at 50°C. |
| Ligations | After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
| Storage conditions | 20 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; glycerol; Store at -20°C. |
| Optimal temperature | 50°C |
| Optimal buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA |
| Assayed on | Lambda DNA |
| Source | Arthrobacter citreus |
| Recognition sites | R↑AATTY |
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