| Campatible Ends | AvaI, BcoI , BmeT110I, BsiHKCI, BsoBI, Eco88I, NspIII |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Methylation sensitivity not tested |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
| Ligations | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Storage conditions | 10 mM KH2PO4 (pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
| Optimal temperature | 37°C |
| Optimal buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA |
| Assayed on | Lambda DNA |
| Source | Alteromonas macleodii 87 |
| Recognition sites | C↑YCGRG |
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