| Campatible Ends | Bsp120I^, PspOMI^ |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Blocked by overlapping Dcm methylation(CmCWGG):GGGCCCWGG |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C |
| Ligations | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C |
| Optimal temperature | 37°C |
| Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA |
| Assayed on | Lambda DNA |
| Source | Acetobacter pasteurianus |
| Recognition sites | GGGCC↑C |
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