| Campatible Ends | NdeI |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Methlation effect | Methylation sensitivity not tested |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
| Ligations | After 10-fold overdigestion with enzyme 80% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better. |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 1mM DTT; 200 μg/ml BSA, 50% glycerol; Store at -20°C. |
| Optimal temperature | 37°C |
| Optimal buffer | Y?(33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) +?BSA |
| Assayed on | Lambda DNA |
| Source | E.coli strain that carries the cloned FauND I gene from Flavobacterium aquatili ND |
| Recognition sites | CA↑TATG |
021-37772169 800-820-1016 400-821-0268 
021-37772170 
sales@sangon.com