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| 产品名称 | 数量 | 现价 | 小计 |
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合计:¥ .00
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| Order NO.: BF0005-2000U | 品牌 : BBI |
| 英文名 | M-MuLV Reverse Transcriptase | |
| 别名 | B600005-0002 | |
| 储存 | All components should be transported at 4°C and stored at -20°C for long time. | |
| 相关类别 | 反转录酶 |
| 产品编号 | 新编号 | 包装规格 | 库存 | 目录价（元） | 网购价（元） | 订购数量 |
| BF0005-10000U | B600005-0010 | 10000 U | 有 | 400.0 | 320.0 | |
| BF0005-2000U | B600005-0002 | 2000 U | 有 | 100.0 | 80.0 | |
| 大包装请询 | ||||||
| Unit definition |
| One unit of the enzyme incorporates 1 nmol of dTMP into a polynucleotide fraction (adsorbed on DE-81) in 10 min at 37°C. |
| Applications |
| First strand cDNA synthesis for RT-PCR and real-time RT-PCR Synthesis of cDNA for cloning and expression Generation of labeled cDNA probes for microarrays DNA labeling Analysis of RNA by primer extension |
| Features |
| Efficient synthesis of full-length first strand cDNA up to 13 kb Optimum activity at 42°C Active up to 50°C Incorporates modified nucleotides |
| General Description |
| M-MuLV Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase RNase H Minus and function in a wide range of temperature（42~55°C）. |
| Properties |
| inhibitor | Metal chelators, inorganic phosphate, pyrophosphate and polyamines |
| 5 × M-MuLV RT buffer | 250 mM Tris-HCl (pH8.3, 25°C), 250 mM KCl, 20 mM MgCl2, 50 mM DTT |
| Unit definition | One unit of the enzyme incorporates 1 nmol of dTMP into a polynucleotide fraction (adsorbed on DE-81) in 10 min at 37°C. |
| Storage buffer | 50 mM Tris-HCl (pH7.5), 0.1 M NaCl, 1 mM EDTA, 5 mM DTT, 0.1%（v/v）Triton X-100 and 50%（v/v）glycerol |
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