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| 产品名称 | 数量 | 现价 | 小计 |
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合计:¥ .00
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| Order NO.: BF0009-2500U | 品牌 : BBI |
| 英文名 | T4 DNA ligase | |
| 别名 | B600009-0003 | |
| 储存 | -20°C | |
| 相关类别 | 限制性内切酶 |
| 产品编号 | 新编号 | 包装规格 | 库存 | 目录价（元） | 网购价（元） | 订购数量 |
| BF0009-1000U | B600009-0001 | 1000 U | 有 | 400.0 | 320.0 | |
| BF0009-2500U | B600009-0003 | 2500 U | 有 | 800.0 | 640.0 | |
| 大包装请询 | ||||||
| Unit definition |
| One unit is the amount of the enzyme catalyzes that conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 minutes at 37°C (4). |
| Applications |
| Cloning of restriction enzyme generated DNA fragments Cloning of PCR products Joining of double-stranded oligonucleotide linkers or adaptors to DNA Site-directed mutagenesis Amplified fragment length polymorphism (AFLP) Ligase-mediated RNA detection Nick repair in duplex DNA, RNA or DNA/RNA hybrids Self-circularization of linear DNA. |
| Features |
| Active in restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky-end ligation is completed in 10 minutes at room temperature Supplied with PEG solution for efficient blunt-end ligation |
| General Description |
| T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.The T4 DNA Ligase requires ATP as a cofactor. |
| Properties |
| Quality Control | The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases confirmed by appropriate quality tests. |
| Source | E.coli cells with a cloned gene 30 of bacteriophage T4 |
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021-37772170 
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