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| 产品名称 | 数量 | 现价 | 小计 |
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合计:¥ .00
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| Order NO.: BF0010-200U | 品牌 : BBI |
| 英文名 | Klenow Fragment | |
| 别名 | B600010-0200 | |
| 储存 | -20°C | |
| 相关类别 | 限制性内切酶 |
| 产品编号 | 新编号 | 包装规格 | 库存 | 目录价（元） | 网购价（元） | 订购数量 |
| BF0010-200U | B600010-0200 | 200 U | 无 | 140.0 | 112.0 | |
| 大包装请询 | ||||||
| Unit definition |
| Unit One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30minutes at 37°C, using poly(dA-dT)·poly(dA-dT) as a template·primer. |
| Applications |
| DNA blunting by fill-in 5'-overhangs Random-primed DNA labeling Labeling by fill-in 5'-overhangs of dsDNA |
| Features |
| Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers |
| General Description |
| Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I. |
| Properties |
| Quality Control | The absence of endodeoxyribonucleases confirmed by appropriate quality test.|Functionally tested for fill in of 5'-overhanging DNA termini and for random primed DNA labeling. |
| Source | E.coli cells with a cloned fragment of the polA gene |
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