user manual, closure plate membrane, sealed bags, coated ELISA plates , Standard, Standard Dilution Buffer, Streptavidin-HRP, Biotinylated anti –Tumor necrosis factor α, TNF-α --antibody, Chromogenic agent Solution A, chromogenic agent Solution B, Stop Solution, wash solution
Applications
This kit allows for the determination of AngⅡ concentrations in Human serum, cell culture supernatant, and other biological fluids.
General Description
The kit assay Human AngⅡ level in the sample，add Human AngⅡ antibody to microtiter plate wells, after Incubating,add Biotinylated anti–AngⅡ -antibody , then Combined Streptavidin-HRP, become complex, after Incubating and washing Completely, Add TMB substrate solution,TMB substrate becomes blue color, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AngⅡ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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